FK506-binding protein 2 (FKBP13) inhibit Bax-induced apoptosis in Saccharomyces cerevisiae (yeast)

Abstract FK506-binding protein 2 (FKBP13) is a part of the immunophilin family involved in immunoregulation. It also believed to operate as factor membrane cytoskeletal framework and an ER chaperone. FKBP2 FKBP1 (FKBP12), known immunophilins, are binding proteins for rapamycin FK506, which immunosuppressive drugs. was suggested that immunophilin-like play significant roles regulating intracellular calcium folding/sorting, acting molecular chaperones. Within 15 mammalian FKBPs known, merely only one proven form complexes with FK506 cytosol facilitate their T cells effects, luminal endoplasmic reticulum (ER) reported take folding ER. However, little about link apoptosis (either pro or anti-apoptotic protein). In this study, FKPB2 co-expressed pro-apoptotic Bax after yeast-based human hippocampal cDNA library screening. The yeast strain carrying gene transformed episomal 2-micron plasmid encodes HA-tagged gene. resultant would allow co-expression cells. results presented here show can role protecting cell from Bax-induced apoptosis.